Isolation and identification of bioflocculant producing stain and analysis on its flocculating characteristics | 生物絮凝剂生产菌的分离与鉴定及其絮凝特性分析
2011
Yang Jinshui, China Agricultural University, Beijing（China）, College of Biological Science | Ma Long, China Agricultural University, Beijing（China）, College of Biological Science | Liu Weijie, China Agricultural University, Beijing（China）, College of Biological Science
[Objective] The aim was to indentify a bioflocculatnt producing strain W5 isolated from soil samples and analyze its flocculating characteristics. [Method] The universal primers of bacterial 16S rDNA were used for PCR amplification of its genome; effects of the medium pH, carbon and nitrogen sources on its flocculating activity were analyzed by using single factor method; and infrared spectroscopy was used for functional groups analysis. [Result] The results of homology comparison showed that strain W5 belonging to Gram-negative, long bacilli and had 98.34% of homology with Hymenobacter gelipurpurascens,. In addition, the optimum carbon source, nitrogen source, pH for fermentation condition were glucose, yeast extract and pH 7.04, respectively. Microbe flocculant produced by W5 strain (MBF-W5) was extracted and purified to obtain reddish and flocculent products. Fourier-transform infrared (FTIR) spectroscopy indicated that the major functional groups of MBF-W5 were hydroxyl, carboxyl and amino. [Conclusion] More than 85% of flocculating activity was achieved under the conditions of 150-500 ul bioflocculation dosage,, 5-65 ℃ temperature and pH 2-10.
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