The effect of probiotics on the morphological characteristics of the small intestinal mucosa
2010
Rekiel, A.,Warsaw Univ. of Life Sciences (Poland). Dept. of Animal Breeding | Bielecki, W.,Warsaw Univ. of Life Sciences (Poland). Dept. of Pathology and Veterinary Diagnostics | Wiecek, J.,Warsaw Univ. of Life Sciences (Poland). Dept. of Animal Breeding
The objective of this study was to determine the impact of antibiotics and selected probiotics on the morphological properties of the small intestinal mucosa in growing-finishing pigs. Two production experiments were conducted on 32 hybrid fatteners (16 pigs per experiment - 8 gilts and young hogs each) fed complete diets in a 2-phase system. The animals were clinically healthy and had been treated for parasites prior to fattening. In the first experiment, diets were supplemented with the antibiotic flavomycin (group C1) and the probiotic ToyoCerin (Bacillus toyoi strain, group E1) at the amount of 0.1%. In the second experiment, flavomycin (C2) and Bactocell (Pediococcus acidilactici, MA18/5M strain, E2) at the amount of 0.01% were supplemented. A histological analysis of small intestinal epithelial sections was performed using bax, bcl-x, bcl-2 polyclonal antibodies and Ki-67 and PCNA monoclonal antibodies. Sections of the duodenum, jejunum and ileum were prepared post mortem. Haematoxylin and eosin H-E, histochemical (PAS-Alcian Blue) and immunohistochemical staining procedures were applied. The results of histological, histochemical and immunohistochemical examinations of the small intestinal mucosa indicated that feed additives and probiotics had a varied effect on the morphological characteristics and the proliferation capacity of crypt epithelium. Enterocyte proliferation in crypt epithelium decreased after the administration of probiotics. The absence of significant differences between groups (C1-E1; C2-E2) may suggest that probiotics have no adverse effect on mucosal epithelial cells.
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