Accumulation of arsenic during the growing period of rainbow trout (Oncorhynchus mykiss)
2011
Celechovska, O.,Veterinarni a Farmaceuticka Univ., Brno (Czech Republic). Ustav Biochemie, Chemie a Biofyziky | Harkabusova, V.,Veterinarni a Farmaceuticka Univ., Brno (Czech Republic). Ustav Biochemie, Chemie a Biofyziky | Macharackova, B.,Veterinarni a Farmaceuticka Univ., Brno (Czech Republic). Ustav Biochemie, Chemie a Biofyziky | Vitoulova, E.,Vysoke Uceni Technicke, Brno (Czech Republic). Ustav Chemie Potravin a Biotechnologii | Lavickova, A.,Veterinarni a Farmaceuticka Univ., Brno (Czech Republic). Ustav Biochemie, Chemie a Biofyziky
Rainbow trout (Oncorhynchus mykiss) was monitored for the duration of one year, from 2 to 14 months of age (n = 120). The study was performed on a farm with through-running water from the basin of water processing plant in the years 2007 and 2008. During the first months of fish life, we analyzed homogenized samples (n = 12) of rainbow trout bodies. From the 8th month the samples of twelve rainbow trouts were analysed. Total As and arsenobetaine concentrations were quantified in muscle, liver, spleen and bile separately. The total As was determined by hydride technique AAS and arsenobetaine by high performance liquid chromatography combined with detection using atomic fluorescence spectrometry. As conc. in homogenized body samples grew significantly during the first months of life, from the 6th month of rearing no significant difference was found. Arsenobetaine conc. in homogenized bodies was low during the first months and from the 6th month it stayed on invariable level and amounted to 46+/-6% of total As. The highest As and arsenobetaine levels in tissues ranked in the order muscle, liver, spleen and bile. In summer months the total As in muscle was bonded in the form of arsenobetaine, in winter months the level of arsenobetaine dropped to 50% of total As. As conc. in liver samples remained the same during the entire monitoring, however, the ratio of arsenobetaine conc. altered. In summer months it reached the maximum 27%, in winter months it dropped to 7% of As. As conc. in spleen and bile decreased in winter months, arsenobetaine conc. corresponded to the value assessed in liver. This study proved the effect of the small content of As in feedstuff on accumulation of total As and arsenobetaine in the tissue of rainbow trout during the growing period and season.
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