Validation of SNP [Single Nucleotide Polymorphism] ACCA: c.5634T>C effects on fatty acid composition in purebred and crossbred Iberian pigs | Validación de los efectos del SNP [Polimorfismo de Nucleótido Simple] ACCA: c.5634T>C sobre la composición de ácidos grasos en cerdos ibéricos puros y cruzados con Duroc
2011
Muñoz, M., Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria, Madrid (España). Dept. de Mejora Genética Animal | Rodríguez, M.C., Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria, Madrid (España). Dept. de Mejora Genética Animal | Fernández, A., Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria, Madrid (España). Dept. de Mejora Genética Animal | Barragán, C., Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria, Madrid (España). Dept. de Mejora Genética Animal | Alves, E., Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria, Madrid (España). Dept. de Mejora Genética Animal | Silió, L., Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria, Madrid (España). Dept. de Mejora Genética Animal
Fatty acid composition influences fat and meat quality as well as human health. In previous works, two QTLs on SSC12 with different effects on fatty acids of subcutaneous and intramuscular fat was reported in F2 (QTLA, backfat fatty acids), F3 and two backcrosses (QTLB, intramuscular fatty acids) of an experimental Iberian x Landrace cross (IBMAP). Moreover, ACACA was tested as functional and positional candidate gene and highly significant association was detected for ACACA SNP: c.5634T>C. In the present work genotyping of SNP c.5634T>C was carried out on purebred Iberian and crossbred Duroc x Iberian heavy pigs, in order to verify the analyzed polymorphism effects. Moreover, effects of other ACACA polymorphisms were analyzed in the purebred pigs. Significant effects of ACACA SNP: c.5634T>C were detected on the percentage of several fatty acids but, the effects differ in both populations, and also are different from those detected in the IBMAP cross in the two analyzed tissues. These results suggest that the polymorphism should be in strong linkage disequilibrium with the causal mutation which could be located into ACACA gene and also support the hypothesis of a tissue dependent genetic control underlying fatty acid metabolism.
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