Deletion of GBG1/AYR1 Alters Cell Wall Biogenesis in Saccharomyces cerevisiae
2010
Ahn, K.W., Chungnam National University, Daejeon, Republic of Korea | Kim, S.W., Chungnam National University, Daejeon, Republic of Korea | Kang, H.G., Chungnam National University, Daejeon, Republic of Korea | Kim, K.H., Chungnam National University, Daejeon, Republic of Korea | Park, Y.H., Chungnam National University, Daejeon, Republic of Korea | Choi, W.J., Ewha Womans University, Seoul, Republic of Korea | Park, H.M., Chungnam National University, Daejeon, Republic of Korea
We identified a gene for β-1,3-glucan synthesis (GBG1), a nonessential gene whose disruption alters cell wall synthesis enzyme activities and cell wall composition. This gene was cloned by functional complementation of defects in β-1,3-glucan synthase activity of the the previously isolated Saccharomyces cerevisiae mutant LP0353, which displays a number of cell wall defects at restrictive temperature. Disruption of the GBG1 gene did not affect cell viability or growth rate, but did cause alterations in cell wall synthesis enzyme activities: reduction of β-1,3-glucan synthase and chitin synthase Ⅲ activities as well as increased chitin synthase Ⅰ and Ⅱ activities. GBG1 disruption also showed altered cell wall composition as well as susceptibility toward cell wall inhibitors such as Zymolyase, Calcofluor white, and Nikkomycin Z. These results indicate that GBG1 plays a role in cell wall biogenesis in S. cerevisiae.
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