Omics-Based Analysis of the luxS Mutation in a Clinical Isolate of Escherichia coli O157:H7 in Korea
2010
Kim, J.C., National Institute of Health, Seoul, Republic of Korea | Yoon, J.W., Konkuk University School of Medicine, Seoul, Republic of Korea | Kim, J.B., Yonsei University, Wonju, Republic of Korea | Oh, K.H., National Institute of Health, Seoul, Republic of Korea | Park, M.S., National Institute of Health, Seoul, Republic of Korea | Lee, B.K., National Institute of Health, Seoul, Republic of Korea | Cho, S.H., National Institute of Health, Seoul, Republic of Korea
The purpose of this study was to investigate the relationship between the global regulatory mechanism known as quorum sensing and expression of virulence factors in Escherichia coli O157:H7. A nonpolar luxS deletion was introduced into the chromosome of strain CI03J, a human clinical isolate from South Korea, to create the ΔluxS mutant strain ML03J. Phenotypic characterization of wild-type and mutant strains demonstrated that ML03J had no obvious growth or metabolic defects on 0.2%, glucose LB medium, produced a functionally defective flagellum, and could not utilize sorbose; the biological significance of sorbose utilization is unknown. Omics-based analysis revealed the involvement of LuxS in the transcriptional activation of several flagella/chemotaxis-related genes (flhD; fliA, C, D, S, Z; and cheA, Y, Z), repression of glutamate-dependent acid resistance genes (gadAB), and expression of virulence factors including Shiga toxin, hemolysin, and SepD within the LEE pathogenicity island.
اظهر المزيد [+] اقل [-]الكلمات المفتاحية الخاصة بالمكنز الزراعي (أجروفوك)
المعلومات البيبليوغرافية
تم تزويد هذا السجل من قبل Korea Agricultural Science Digital Library