In Vitro Development of Porcine Parthenogenetic Embryos under the Oil-free Culture System
2010
Park, S.K., Seoul National University School of Dentistry, Seoul, Republic of Korea | Choi, Y.J., Seoul National University College of Medicine, Seoul, Republic of Korea | Roh, S.H., Seoul National University School of Dentistry, Seoul, Republic of Korea
Optimization of the preimplantation mammalian embryo culture condition was widely focused on refining medium composition under the name of chemically defined media. However, recent research revealed that the alteration of physical environment can be a crucial factor to a successful embryo development. In this study, under the same embryo density, a novel culture device named oil-free micro tube culture (MTC) system was evaluated using porcine parthenogenetic embryos. The activated oocytes were placed into the 0.2 ml thin-wall flat cap PCR tube and cultured to the blastocyst stage. As a preliminary step, embryo density and culture medium volume were optimized under a standard drop culture system. The optimal embryo density range for in vitro culture was 0.5 embryos per μl in 20 μl drop (20.5%) and 1.0 embryos per μl in 10 μl drop (20.6%). Based on these results, we compared drop culture system and 'MTC' system in terms of the developmental rate to the blastocyst stage. In 20 μl medium volume, the 'MTC' system showed similar blastocyst formation rate when compared with drop culture system (20.2% versus 20.5%, respectively) while the 'MTC' system showed lower blastocyst formation rate than drop culture system in 10 μl one (12.7% versus 20.0%, respectively). Therefore the 20 μl MTC system may be an alternative incubation system for short-distance embryo transport without carrying the CO₂ incubator and this provides novel embryo culture device to clinical veterinary embryologists.
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