Mass rearing of Cotesia sp. and its parasitization of Diaphenea indica infesting ampalaya
2009
Sandoval, R.J.C. | Carrido, D.R. | Ocampo, V.R., Philippines Univ. Los Baños, College, Laguna (Philippines). Crop Protection Cluster
The host insect, Diaphania indica (Saunders), is being reared in the laboratory using the mass rearing procedures of Cabriga (1996) with slight modification. Pupae of Cotesia sp. were collected from cucurbit fields and placed in rearing cages in the laboratory. Upon emergence of the adult wasp, honey solution in a cotton wad was placed in the container and Diaphania indica larvae niched in Luffa leaves were then exposed to the parasitoid for 24 hours. Then the larvae were transferred to 750 ml plastic containers, lined with paper and provided daily with fresh Luffa leaves. The paper linings were replaced only when necessary to limit both the disturbance to the larvae and the exposure to microbial contamination. Once the host larvae reached 5th instar, strips of paper were provided in the container for parasitoid pupation. The Cotesia pupae were then collected using a fine paintbrush and placed in the plastic bottle for adult emergence.
اظهر المزيد [+] اقل [-]الكلمات المفتاحية الخاصة بالمكنز الزراعي (أجروفوك)
المعلومات البيبليوغرافية
تم تزويد هذا السجل من قبل University of the Philippines at Los Baños