Morphological and molecular identification of Colletotrichum acutatum from tomato fruit
2010
Živković, S., Institute for Plant Protection and Environment, Belgrade (Serbia) | Stojanović, S., Institute for Plant Protection and Environment, Belgrade (Serbia) | Ivanović, Ž., Institute for Plant Protection and Environment, Belgrade (Serbia) | Trkulja, N., Institute for Plant Protection and Environment, Belgrade (Serbia) | Dolovac, N., Institute for Plant Protection and Environment, Belgrade (Serbia) | Aleksić, G., Institute for Plant Protection and Environment, Belgrade (Serbia) | Balaž, J., Faculty of Agriculture, Novi Sad (Serbia)
In Serbia, the occurrence of anthracnose on tomato fruit has been recorded during the last several years. Typical fruit symptoms include dark, sunken, and circular lesions with orange colonial masses. Pathogen isolates were obtained from a diseased tomato fruits, on PDA medium forming a white to gray colonies. The cultures developed black acervuli around the center of the colony. Conidia were hyaline, aseptate, and fusiform or rarely cylindrical. Appressoria were smooth, simple, clavate to ovate, and varied from light to dark brown. Pathogenicity tests with representative isolates were conducted on symptomless, detached tomato fruits. All tested isolates caused anthracnose lesions on tomato fruit after 7 days of incubation. Koch's postulates were fulfilled by reisolation from incubated tomato fruits. PCR analysis of genomic DNA from tomato isolates resulted in an amplification product of 490 bp, specific for C. acutatum, further confirming the identity of the pathogen. Based on morphological and molecular characteristics, the isolates from tomato fruit were determined as Colletotrichuma acutatum
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