Comparative investigations of sensitivity and specificity of immunoenzime probe and inhibition hemagglutination test in serological diagnostics of Newcastle disease in poultry | Uporedno ispitivanje osetljivosti i specifičnosti imunoenzimske probe i testa inhibicije hemaglutinacije u serološkoj dijagnostici Newcastle bolesti živine
2009
Milić, N., Fakultet veterinarske medicine, Beograd (Serbia) | Nišavić, J., Fakultet veterinarske medicine, Beograd (Serbia) | Radojičić, M., Fakultet veterinarske medicine, Beograd (Serbia) | Šekler, M., Veterinarski specijalistički institut Kraljevo, Kraljevo (Serbia) | Matović, K., Veterinarski specijalistički institut Kraljevo, Kraljevo (Serbia) | Vidanović, D., Veterinarski specijalistički institut Kraljevo, Kraljevo (Serbia)
Comparative investigations of the sensitivity and specificity of the indirect immunoenzime probe - iELISA and the hemagglutination inhibition test (HI test) in serological diagnostics of the Newcastle disease in poultry were carried out using samples of blood serum taken from non-vaccinated and vaccinated poultry. A total of 14 samples of blood serum from non-vaccinated poultry were examined using the immunoenzime probe - iELISA, and nine of these were found to be positive to the presence of specific antigen against the Newcastle disease virus, while two samples were suspect, and no presence of specific antibodies was established in three samples. Examinations of 82 samples of blood serum from vaccinated poultry for the presence of specific antibodies against Newcastle disease virus established their presence in 80 serum samples, while one sample was suspect and one sample was negative. The values of the titer of specific antibodies in blood serum samples of vaccinated and non-vaccinated poultry established using the hemagglutination inhibition test (HI test) ranged from 1:2 to 1:32.
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