Characteristics of Pseudomonas syringae strains originating from a pear fruit trees in Serbia | Karakteristike izolata Pseudomonas syringae izolovanih sa kruške u Srbiji
2009
Gavrilović, V., Institut za zaštitu bilja i životnu sredinu, Beograd (Serbia) | Ivanović, Ž., Institut za zaštitu bilja i životnu sredinu, Beograd (Serbia) | Živković, S., Institut za zaštitu bilja i životnu sredinu, Beograd (Serbia) | Savić, D., Agromarket d.o.o., Kragujevac (Serbia)
The test results of Pseudomonas syringae strains, isolated by a pear trees are as given. The symptoms of disesease, caused by this bacterium, appeared in two types: a blossom blast, a trunk necrosis and a branch followed by canker formation. All strains are Gram negative, fluorescent on a King medium B and oxydative (O/F test). The tested strains are HR positive, producing a levan, but don't oxydase, arginindehydrolase and pectinase (LOPAT +---+). Strains originated from a pear trees caused necrosis on an artificial inoculated pear, cherry and lemon fruits, as well as a syringae leaves and bean pods. The results of differential tests for P. syringae pv. syringae and P. syringae pv. morsprunorum (GATT) revealed that the tested strains hydrolise gelatin and esculin, but the negative results are recorded in tyrosinase production and tartrate utilization tests. The PCR analysis, by using a BOX primer, shows the high level of symilarity among the Serbian P. syringae strains, isolated from a pear fruit trees, but also within a slice differencies, compared with a check strain CFBP 1582. These results confirm a previous data about genetic diversity among P. syringae strains originated from a different areas all around the world. Based on the obtained results, it's concluded that the tested strains belong to P. syringae pv. syringae. Further characterization of P. syringae strains, isolated from pear in Serbia use the ERIC and REP PCR and it's still underway.
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