Identification of Tilletia species using rep-PCR fingerprinting technique
2011
Župunski, V., Maize Research Institute Zemun Polje, Belgrade - Zemun (Serbia) | Ignjatović-Micić, D., Maize Research Institute Zemun Polje, Belgrade - Zemun (Serbia) | Nikolić, A., Maize Research Institute Zemun Polje, Belgrade - Zemun (Serbia) | Stanković, S., Maize Research Institute Zemun Polje, Belgrade - Zemun (Serbia) | Jevtić, R., Institute of Field and Vegetable Crops, Novi Sad (Serbia) | Lević, J., Maize Research Institute Zemun Polje, Belgrade - Zemun (Serbia) | Ivanović, D., Maize Research Institute Zemun Polje, Belgrade - Zemun (Serbia)
Analyzing 167 non-processed seed samples of wheat, it was found that 145 samples (86.8 %) were contaminated with Tilletia species, while 22 (13.2 %) samples were not contaminated. By using rep-PCR fingerprinting technique, it was found that DNA isolates of T. tritici originated from Serbian wheat samples had 80 % similarity with positive control for T. tritici. One isolate shared similarity of 60% with T. tritici, T. controversa and T. laevis. It was supposed that this isolate belongs to T. bromi. Isolate of T. laevis shared a similarity of 70 % with isolates of T. tritici and T. controversa, while T. walkeri was more than 10 % similar with T. tritici, T. controversa and T. laevis. Although T. controversa and T. tritici had high percent of genetic similarity, they were clustered separately. Our results suggest that rep-PCR fingerprinting could be a useful tool for monitoring presence of morphologically similar Tilletia species in wheat production areas.
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