Pisum and ervilia Tetovac - made in Early Iron Age Leskovac. Part two. Extraction of the ancient DNA from charred seeds from the site of Hissar in South Serbia
2011
Jovanović, Ž., Institute of Molecular Genetics and Genetic Engineering, Belgrade (Serbia) | Stanisavljević, N., Institute of Molecular Genetics and Genetic Engineering | Nikolić, A., Institute of Molecular Genetics and Genetic Engineering, Belgrade (Serbia) | Medović, A, Museum of Vojvodina, Novi Sad (Serbia) | Mikić, A, Institute of Field and Vegetable Crops, Novi Sad (Serbia) | Radović, S., Faculty of Biology, Belgrade (Serbia) | Đorđević, V., Institute of Field and Vegetable Crops, Novi Sad (Serbia)
The extracts were prepared from the samples of 3,200-year-old charred pea and bitter vetch seeds from the site of Hissar near Leskovac, South Serbia, using two different DNA extraction procedures. We used CTAB method with some modification and obtained low quantity of ancient DNA in comparison with the second method used - commercial available kit. After the extraction, a whole genome amplification using Phi29 DNA polymerase was performed. The amplified DNAs were used for PCR reaction using primers for 26S rDNA gene, which is located on the nuclear genome. The single band corresponding to 26S rDNA fragment from modern relatives was obtained. We conclude that DNA from charred pea and vetch seed can be extracted and used for further archaeobotanical analysis at the molecular level.
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