RAPD markers in estimating diversity of soybean parental genotypes and predicting traits of progeny
2008
Perić, V., Maize Research Institute Zemun Polje, Belgrade-Zemun (Serbia) | Mladenović-Drinić, S., Maize Research Institute Zemun Polje, Belgrade - Zemun (Serbia) | Srebrić, M., Maize Research Institute Zemun Polje, Belgrade - Zemun (Serbia)
Objection of this study was to estimate genetic distance (GD) between genotype ZPS 015 and genotypes L 91-3103, Ravnica, Shine, L 90-14 and ZPS 107, and compare GD to seed yield and number of superior lines of their progeny. Genotype ZPS 015 has been used as a parent in crosses with other five genotypes. RAPD analysis of these genotypes with 18 random primers gave in total 75 fragments which of 20% were polymorphic. Coefficients of similarity were calculated by Jaccard, Rogers and Tanimoto, Sorensen-Dice and Sokal and Michener. All coefficients showed small genetic distance between parental lines. Low level of polymorphism and genetic divergence between parental pairs is in accordance with previous analyses about polymorphism of soybean, as a species with very narrow genetic background. In order to compare genetic distance between parents and seed yields data of F4 progeny by each cross, Spearmans Rank Correlation Coefficient was calculated. This coefficient showed negative and non-significant correlation.
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