Biochemical characteristics of the Aeromonas species isolated from the trout | Biohemijske karakteristike Aeromonas vrsta izolovanih iz pastrmke
2011
Đorđević, V., Institut za higijenu i tehnologiju mesa, Beograd (Serbia) | Baltić, M.Ž., Fakultet veterinarske medicine, Beograd (Serbia) | Ćirković, M., Poljoprivredni fakultet, Novi Sad (Serbia) | Milošević, N., Poljoprivredni fakultet, Novi Sad (Serbia) | Mitrović, R., Institut za higijenu i tehnologiju mesa, Beograd (Serbia) | Đurić, J., Fakultet veterinarske medicine, Beograd (Serbia)
The presence of bacteria of Aeromonas spp. in samples of skin and muscles of trout were investigated. Biochemical properties of isolated species of bacteria were tested using conventional methods of microbiological diagnostics and commercial tests API 20 E (bioMerieux). For the purpose of this study, trout was stored in freezer on crushed ice – superchilling in ratio 50% : 50%. For isolation of bacteria of Aeromonas spp., fish skin (surface of 17 x 2 square cm) and muscle (mass of 25 g) were used. Total of 12 bacteria strains from Aeromonas spp. were isolated, 7 (58.3%) belonged to strain A. hydrophila group 1, and 5 (41.6%) belonged to strain A hydrophila group 2. In identification of isolated bacteria of the Aeromonas spp. it was established that 91.67% of isolates had beta galactosidase, were citrate positive, indol positive, sorbitol negative, arabinose positive, produced NO2 and reduced it to N2, 83.33% of isolates were Voges-Proskauer positive, melibiose negative, 66.67% of isolates were lysin decarboxylase positive, amigdalin positive, 58.33% of isolates were glucose positive. Mobile Aeromonas species were isolated from studied trout samples, after six days of storage on crushed ice – superchilling. All strains of Aeromonas hydrophila isolated from trout based on biochemical properties corresponded to typical representatives of the species.
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