Studies of Rubus viruses in Slovenia
2011
Mavric-Plesko, I., Agricultural Institute of Slovenia, Ljubljana (Slovenia) | Virscek-Marn, M., Agricultural Institute of Slovenia, Ljubljana (Slovenia) | Koron, D., Agricultural Institute of Slovenia, Ljubljana (Slovenia)
The number of viruses found in raspberries and blackberries has increased greatly in last years. The study of Rubus viruses in Slovenia started in 2003. Symptoms of raspberry bushy dwarf virus (RBDV) were observed on some red raspberry cultivars in collection plantation of Agricultural Institute of Slovenia. All red raspberry accessions were tested for RBDV infection by DAS-ELISA. The infection with RBDV was confirmed in some wild and commercial accessions in the collection. RBDV was found also in grapevine and further studies of variability and transmission of the virus were initiated. Monoclonal antibodies were used to serologically characterize selected isolates. RNA-2 of selected isolates was amplified by RT-PCR and sequenced. Serological and molecular differences were observed between isolates from both hosts as well as between the isolates from the same host. Red raspberry and blackberry accessions in the collection were tested by DAS-ELISA for infection with several nepoviruses. They were found not to be infected with nepoviruses. Total RNA was isolated from blackberry plant showing virus-like symptoms. Using RT-PCR sample was tested for the presence of several viruses which are known to infect blackberries. Amplification products were obtained only with primers for Rubus yellow net virus (RYNV). Amplification products were sequenced and the sequences confirmed the infection with RYNV. Further testing of cultivated and wild Rubus sp. for virus infections is planned for future.
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