Preparation and application of recombinant PmRab7 in shrimp farm
2010
Natapong Jupatanakul(Mahidol University, Bangkok (Thailand). Faculty of Science. Department of Biotechnology) E-mail:[email protected] | Flegel, Timothy W.(Mahidol University, Bangkok (Thailand). Faculty of Science. Centex Shrimp) | Boonsirm Withyachumnarnkul(Mahidol University, Bangkok (Thailand). Faculty of Science. Centex Shrimp) | Kallaya Sritunyalucksana(Mahidol University, Bangkok (Thailand). Faculty of Science. Centex Shrimp)
White spot syndrome virus (WSSV) is the most serious pathogen in shrimp aquaculture costing billions of dollars in Thai export earnings. Recent study found a shrimp molecule called PmRab7 was bound to the viral envelope protein, VP28. This suggests the potential use of PmRab7 for WSSV protection in shrimp aquaculture management. The Pichia pastoris expression system was used in PmRab7 production for farm trials by oral administration. In the field trial, 1 mg and 2 mg PmRab7/kg shrimp feed were used for WSSV protection. Both doses helped to reduce the mortality resulting from the WSSV challenge. On day 4 after the WSSV was challenged, mortality rate in the control group was 65-80 percent, while that for the PmRab7-feed group was 20-40 percent CM. However, after cessation of PmRab7 feeding for 3 days, there was no significant difference in mortality (P GT 0.05) between the control and PmRab7-supplemented group. Nevertheless, challenged shrimp eventually died after one week. Low quality of feed pellet was the problem found in test, therefore, top-dressing technique was used instead. With this technique, PmRab7-containing yeast could stable in shrimp feed pellet for at least 2 hours. We hope our work could help in sustainable disease prevention in shrimp industry.
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