Production of transgenic pig clone embryo expressed the red fluorescent protein by using the somatic cell nucleus transplantation technology | ╃ㄤ缁哥Щ妞浜ц〃杈剧孩茶у界浆哄
2011
Kang Jindan, Yanbian University, Yanji China锛, Institute of Animal Embryo Engineering | Yin Xijun, Yanbian University, Yanji China锛, Institute of Animal Embryo Engineering | Zhao Minghui, Yanbian University, Yanji China锛, Institute of Animal Embryo Engineering
[Objective] The research aimed to lay the foundation for producing the transgenic clone pig. [Method] The pig fetus fibroblast with the red fluorescent protein (RFP) gene that was transfected by the retrovirus was as the donor of nucleus transplantation. By using the somatic cell cloning technology, the development situation in vitro of clone embryo with RFP was studied. [Result] The fusion rate of RFP transgenic cell was 83.87% which had no significant difference with 80.56% of non-transgenic cell (P 0.05). The blastula rate in vitro of RFP transgenic somatic cell reconstructed embryo was 8.67% which had no significant difference with 6.56% of non-transgenic cell (P 0.05). After the reconstructed embryo of RFP transgenic somatic cell was transplanted into fifteen receptors, there was no conception individual. [Conclusion] The transgenic cell with the red fluorescent protein as the donor could successfully clone the transgenic embryo and obtain the transgenic blastula.
اظهر المزيد [+] اقل [-]الكلمات المفتاحية الخاصة بالمكنز الزراعي (أجروفوك)
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