IP3 sensitive calcium channel involved in the jasmonic acid induced calcium mobilization | IP3 绂诲ч涓歌瀵肩ㄥ
2011
Li Yangyang, Beijing University of Agriculture, Beijing (China), College of Biotechnology | Yu Yongkun, Beijing University of Agriculture, Beijing (China), College of Biotechnology | Wan Shanxia, Beijing University of Agriculture, Beijing (China), College of Biotechnology
[Objective] This study was to investigate the role of IP3 sensitive calcium channel in the JA-induced calcium mobilization pathway. [Method] Arabidopsis thaliana leaves were labeled by Fluorescent probe Fluo-3/AM under low temperature at 4 to measure the fluorescent intensity of intracellular Ca2+ which was pretreated with heparin on jasmonic acid (JA)-induced. [Results] When A. thaliana leaf cells were pretreated with 10, 50 or 100 ng/ml heparin, intercellular free Ca2+ fluorescence intensity was reduced in comparison with negative control. Once the heparin-pretreated A. thaliana leaf cells were stimulated with 100 渭mol/L JA, intercellular Ca2+ fluorescence intensity increased gradually and tended to be stable at a degree equivalent with that in negative control. [Conclusion] The experiment showed that the pretreatment with heparin could inhibit the increase of the intracellular Ca2+ concentration significantly which JA-induced in leaves of Arabidopsis thaliana.
اظهر المزيد [+] اقل [-]الكلمات المفتاحية الخاصة بالمكنز الزراعي (أجروفوك)
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