Validation of a simple method for the interpretation of uterine cytology in cows
2012
Prieto, M., University of Santiago de Compostela, Lugo (Spain). Unit of Reproduction and Obstetrics | Barrio, M., University of Santiago de Compostela, Lugo (Spain). Unit of Reproduction and Obstetrics | Quintela, L.A., University of Santiago de Compostela, Lugo (Spain). Unit of Reproduction and Obstetrics | Perez-Marin, C.C., University of Cordoba (Spain). Dept. of Animal Medicine and Surgery | Becerra, J.J., University of Santiago de Compostela, Lugo (Spain). Unit of Reproduction and Obstetrics | Vigo, M., University of Santiago de Compostela, Lugo (Spain). Unit of Reproduction and Obstetrics | Diaz, C., University of Santiago de Compostela, Lugo (Spain). Unit of Reproduction and Obstetrics | Cainzos, J., University of Santiago de Compostela, Lugo (Spain). Unit of Reproduction and Obstetrics | Prieto, A., University of Santiago de Compostela, Lugo (Spain). Unit of Reproduction and Obstetrics | Fernandez, F.I., FEFRIGA, A Coruna (Spain). Unit of Embryo Transfer | Martinez, D., FEFRIGA, A Coruna (Spain). Unit of Embryo Transfer | Herradon, P.G., University of Santiago de Compostela, Lugo (Spain). Unit of Reproduction and Obstetrics
One of the main drawbacks of using endometrial cytology in cows is the time required for sample collection and interpretation. It is recommended to count a large number of polymorphonuclear neutrophils (PMN) and to calculate their overall percentage. However, since counting a large number of cells is a laborious method, it would be preferable to simplify the analysis by counting the number of PMN in few microscopic fields. Therefore, the aim of this study was to assess whether a simple test, based on calculating the average number of PMN in 10 fields at 1000x, could be a reliable technique for the diagnosis of endometritis. Two hundred and sixty endometrial samples were taken from Holstein cows at different postpartum stages using an adapted cytobrush. Smears obtained were air-dried for fixing and stained with a Romanowsky-type procedure. To evaluate the counting method, the percentage of PMN in 150 cells was calculated as well as the average number of PMN in 10 fields at 1000x. Receiver operating characteristic curves were constructed to evaluate both methods, the percentage of PMN (used as reference) and the average number of PMN. It was observed that the area under the curve is (regardless of cut-off used) higher than 0.99 and the correspondences between both methods were 1.58 PMN/field for the cut-off value of 15% and 2.40 PMN/field for the cut-off value of 20%. These results show that this simple method could be used to determine the percentage of PMN in endometrial cytological samples and to diagnose endometritis in cows.
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