Using of cells and their organelles as tools in study the toxic effect of deltamethrin and profenofos

The purpose of the present study is to investigation the potential toxic effects of deltamethrin and profenofos (1/10 and 1/20 of LD5a) that have different active ingredient on blood picture and ultrastructure changes on cell's contents of liver and Kidney as tools in study the toxic effects on albino rats. Obtained data indicated that deltamethrin and profenofos at 1/10 and 1/20 of LD50 caused increase in WBC's count after nine months. While the two formulations caused significant decrease in RBC's count after same time , similar effects were noticed on Hemoglobin value, and platelets count of treated rat. No sharp variations were noticed between different two formulations and different doses in cellular organelles in liver cells treated rats. The results so far obtained indicated histo-and cytopathological alteration by electron microscope due to the effect of compounds when compared with control ones, some sections poorly differentiated hepatocellular showing marked polymorphis of the nuclei, irregularity of the chromatin and prominent nucleoli in treated rats with deltamethrin when compared with control. Also, in treated with profenofos showed numbers of nuclei and their content in different section by many powers of vision, results show differences and all treatments induce genotoxicity. Kidney sections of different pesticides revealed many phases of degeneration changes when compared with those of control. Electron microscope appeared differentiated cells containing a cytoplasmic indentation or inclusion filled with brush border material and showed also, that cytoplasm is filled with prominent granular, endoplasmic reticulum containing some dense flocculam materials. Also, electron micrograph appeared differentiated in nuclei and changed in their content such as nucleolus, and chromosomes (chromatid and centromere) so, from the dose applied during nine months results conclude that all tested pesticides show genotoxicity in treated animals.