The virulence of Brucella abortus isolated from cattle in Korea
2011
Lim, J.J., Gyeongsang National University, Jinju, Republic of Korea | Kim, J.H., Northern Branch, Gyeongbuk Veterinary Service Laboratory, Andong, Republic of Korea | Kim, D.H., Gyeongsang National University, Jinju, Republic of Korea | Lee, J.J., Gyeongsang National University, Jinju, Republic of Korea | Kim, D.G., Gyeongsang National University, Jinju, Republic of Korea | Jun, M.H., Chungnam National University, Daejeon, Republic of Korea | Kim, S.H., Chungnam National University, Daejeon, Republic of Korea | Chang, H.H., Gyeongsang National University, Jinju, Republic of Korea | Lee, H.J., Gyeongsang National University, Jinju, Republic of Korea | Min, W.G., Gyeongsang National University, Jinju, Republic of Korea | Kim, S., Gyeongsang National University, Jinju, Republic of Korea
In this study, we isolated 12 of Brucella (B.) spp. from cattle, which have been positive in Rose Bangal test and tube agglutination test in Gyeongbuk province in 2009. According to AMOS PCR analysis, isolated 12 strains were identified as B. abortus. Murine derived macrophage, RAW 264.7 cells, were infected with isolated 12 strains or reference strain (B. abortus 544), and bacterial internalization were characterized. According to these results, we divided the isolated strains into the following three groups: class Ⅰ, lower internalization than that of B. abortus 544; class Ⅱ, similar internalization to that of that of B. abortus 544; class Ⅲ, higher internalization than that of B. abortus 544 within RAW 264.7 cells. Furthermore, intracellular growth, bacterial adherent assay, LAMP-1 colocalization, virulence in mice and surface protein pattern were characterized. From these results, representative strains of class Ⅲ showed lower LAMP-1 colocalization, higher adherent efficiency, higher virulence in mice than those of B. abortus 544, and showed different pattern of surface proteins. These results suggest that B. abortus field strains, isolated from cattle in Korea, possess various virulence properties and higher internalization ability of field strain may have an important role for its virulence expression.
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