Movement and multiplication of Spiroplasma kunkelii in corn.
1995
Gussie J.S. | Fletcher J. | Claypool P.L.
After the introduction of Spiroplasma kunkelii into the leaves of corn (Zea mays) seedlings by inoculative corn leafhoppers (Dalbulus maidis), the pathogen was detected by enzyme-linked immunosorbent assay. Spiroplasmas were detected in leaves 14 days after exposure to inoculative leafhoppers (as much as 2 weeks before symptoms appeared) and in roots after 20 days. The probabilities of detecting spiroplasmas in different plant organs, determined by logistic regression, generally increased over time; the probability was greater in roots than in leaves at most testing dates. Spiroplasmas moved into tassels as they developed. The age and/or size of the plant at the time of infection affected pathogen movement; spiroplasmas were detected earlier in roots of plants inoculated at the one-leaf seedling stage than in those of plants inoculated at the four-leaf stage. Spiroplasma titers remained relatively low in all the plants until the third or fourth week after inoculation. After that time populations in different plant samples varied within a large range, and the upper limit of the range generally increased with time. The maximum titers were reached earlier in plants inoculated at the four-leaf stage than in plants inoculated at the one-leaf stage. Because S. kunkelii may be present in various plant organs well before symptoms appear, spiroplasmas might be acquired by leafhoppers before disease is obvious in the field.
اظهر المزيد [+] اقل [-]الكلمات المفتاحية الخاصة بالمكنز الزراعي (أجروفوك)
المعلومات البيبليوغرافية
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