Preliminary studies on the application of mutagens and in-vitro techniques to rice seeds for inducing salt tolerant mutants.
1988
Liang Zhuqing | Gao Mingwei | Xiang Youbin
The mature seeds of four rice varieties were used as explants in this study. Dehulled rice seeds were treated with gamma-rays irradiation and sodium azide either in combined way or alone. Calli derived from "IR28" and "Zhenshen 97 B" were also irradiated with gamma-rays. The results showed that the callus induction rate and callus growth rate were seriously inhibited when the seeds had been exposed to 15 kR gamma-rays, 1 mM sodium azide (SA) had some stimulative effects on these two parameters. It also indicated that the plantlet regeneration frequency decreased while all the mutagenic treatments employed. The callus growth rate and plantlet regeneration frequency decreased with the increasing of NaCl content in salt enriched selective medium. The concentration of 1% NaCl (W/V) in medium is considered as optimal stress level to identify and isolate salt tolerant callus lines. These lines maintained their capacity of plant regeneration. The optimal radiation dosage for treating callus derived from rice seeds ranges 500-750R.
اظهر المزيد [+] اقل [-]الكلمات المفتاحية الخاصة بالمكنز الزراعي (أجروفوك)
المعلومات البيبليوغرافية
تم تزويد هذا السجل من قبل Wolters Kluwer