Studies on the immobilization of lipase by adsorption method.
1985
Park J.H. | Lee Y.C.
To utilize lipase obtained from Candida cylindracea for lipid hydrolysis, methods to immobilized lipase by adsorption and reaction characteristics of the immobilized lipase by adsorption were investigated. Among the tested adsorbents, silica gel was selected as a suitable adsorbent. The optimum condition for adsorption of lipase was when 47.5 units of lipase were adsorbed to 1.6g of silica gel at pH 7.0 and 5 deg. C for 100 min. Optimum pH and temperature for activity of the immobilized lipase were at 37 deg. C and pH 7.0 which were same as the soluble lipase. Optimum enzyme concentration of the immobilized lipase were 30g for milk fat and 80g for olive oil, whereas those of the soluble lipase were 800 units for milk fat and 1200 units for olive oil.
اظهر المزيد [+] اقل [-]الكلمات المفتاحية الخاصة بالمكنز الزراعي (أجروفوك)
المعلومات البيبليوغرافية
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