Agrobacterium-mediated transformation of sweetpotato (Ipomoea batatas) and regeneration of transformants.
1997
Pido N.L. | Kowyana Y. | Karube M. | Shimonishi K.
A trial was conducted to transform sweetpotato using Agrobacterium tumefaciens, a soil bacterium now widely employed in transferring specific genes to plants. Cotyledon and hypocol pieces of germinated seeds from three crosses were inoculated with A. tumefaciens strain EHA 101 carrying pBIHI-IG, a plasmid which contain within its T-DNA region to genes for kanamycin and hygromycin resistance and Beta-glucuronidase (GUS). When cultured in regeneration medium containing hygromycin, the inoculated explants gave rise to many antibiotic-resistant compact calli which, when subcultured in several antibiotic-free regeneration media, failed to produce shoots. However, when compact calli were induced to form embryogenic calli using picloram, an auxin, the resulting embryogenic calli from the progenies of one cross (Kyushu 111 x Kanto 104) eventually gave rise to over 100 normal-looking whole plants. GUS test and southern hybridization showed that all regenerated plants were solid transformants. Out of eight second-vegetative-cycle transformants now being grown to reprodutive stage, four have already been able to produce flowers.
اظهر المزيد [+] اقل [-]الكلمات المفتاحية الخاصة بالمكنز الزراعي (أجروفوك)
المعلومات البيبليوغرافية
تم تزويد هذا السجل من قبل Wolters Kluwer