Enhanced tolerance of transgenic rice over-expressing Arabidopsis thaliana nucleoside diphosphate kinase(AtNDPK2) against various environmental stresses
2011
Manigbas, N.L., Philippine Rice Research Inst., Science City of Muñoz, Nueva Ecija 3119 (Philippines) | Park, D.S. | Park, S.K. | Han, S.I., Rural Development Administration, Milyang, 627-803 (Korea). Dept. of Functional Crop | Park, H.M., Rural Development Administration, Suwon, 441-855 (Korea) | Hwang, W.H. | Kim, S.M. | Wang, H.J. | Kang, H.W., Rural Development Administration, Milyang, 627-803 (Korea). Dept. of Functional Crop | Kim, D.H., Dong-A Univ., Busan 604-714 (Korea). Coll. of Natural Resources and Life Science | Yun, D.J., Gyeongsang National Univ., Jinju 660-701 (Korea). Div. of Applied Life Science | Yi, G., Rural Development Administration, Milyang, 627-803 (Korea). Dept. of Functional Crop
Multiple tests to assess different abiotic stresses in rice over expressing the Arabipdopsis thaliana Nucleosides Diphosphate Kinase (AtNDPK2) gene have not been extensively studied. The responses of transgenic AtNDPK2 rice lines driven by two different promoters from sweet potato(SWPA2) and cauliflower mosaic virus(CaMV35S) were presented. Transgenic rice lines were evaluated in terms of their tolerance for various abiotic stresses, including cold, drought, ozone, salinity and ultraviolet irradiation. Results indicate that the transgenic lines were highly tolerant of osmotic stress at 20% polyethylene glycol(PEG) and of salt: stress at 100 mM NaCl. The same tolerance was observed with the 5-h continuous UV treatment and ozone. Moderate to weak tolerance for cold and salinity was observed. The results suggest that the AtNDPK2 gene was responsible for the tolerance for various abiotic stresses in rice.
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