Further studies of mitochondrial genome variability in Ponto-Caspian Proterorhinus species (Actinopterygii: Perciformes: Gobiidae) and their taxonomic implications
2011
Sorokin, P.A. | Medvedev, D.A. | Vasil'ev, V.P. | Vasil'eva, E.D.,Moscow State University, Moscow (Russia). Zoological Museum
A new taxonomy of tubenose gobies of the genus Proterorhinus has been proposed recently. The new taxonomy through a phylogeographic analysis of the fish from 15 sites in Southern Ukraine and Russia, through analyzing the genotypes deposited in the GenBank and to define the range of those species was tested. Fin clips were sampled from 52 specimens of tubenose gobies collected from 2003 up to 2009 in 15 localities of the Ponto-Caspian basin. Genomic DNA for amplification of mitochondrial cyt b gene fragment (about 408 bp) was extracted. Tubenose gobies from the Black Sea basin are represented by two euryhaline species historically distributed in different areas: P. semilunaris in the north-western part of the Black Sea basin and P. marmoratus in the north-eastern part. The present data on the variability of the mitochondrial cyt b gene fragment in different tubenose goby populations result in revision of some previous taxonomic hypotheses in this fish group. At the same time further molecular genetic researches of Caspian populations seem to be the main tool for future development of taxonomic conclusions
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