Means of molecular nucleic acids analysis in soil investigations
2011
Wolinska, A. | Stepniewska, Z. | Woloszyn, A. | Pytlak, A. | Dziuba, A.,Katolicki Uniwersytet Lubelski, Lublin (Poland). Katedra Biochemii i Chemii srodowiska,
Soil bacteria consist the huge quantity of genetic information, which constantly remained inaccessible for traditional microbiological tests. That is why, progressive techniques of molecular biology are more and more exploited at investigations of bacterial population in the soil environment. In the current work the most popular molecular methods useful in the soil DNA determination and leading to microbial identification are presented. Choice of the proper molecular method should by anyhow adjusted to the problem, which may be solved; detection, identification, differentiation, taxonomic investigations. Technical aspects are also of great importance; analysis difficulties, experience, research background, costs of method applied
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