Real possibilities for using embryo transfer in cattle genetic improvement | Realne mogućnosti primene embriotransfera za poboljšanje genetike goveda kod nas i u svetu
2011
Dovenski, T., Fakultet za veterinarna medicina, Skopje (The Former Yugoslav Republic of Macedonia)
Multiple Ovulation and Embryo Transfer (MOET) is a biotechnology for accelerating genetic progress in cattle, especially in dairy cows. This technology involves a series of procedures that generate more embryos in one cycle of the donor cow, which are subsequently flushed from the uterus, assesses their quality and transplanted the same day of the heifer recipients or after freezing/thawing procedure. Superovulatory protocol, which stimulates more ovarian follicles to grow simultaneously and ovulate, should be performed by several days application of purified FSH preparations, and synchronization of estrus using PgF2 alpha. Flushing the uterus is carried out by D-PBS medium, 6-8 days after estrus, when the embryos are at the stage of morula or blastocytsts. All embryos with good quality: a spherical shape, with symmetrical cells, uniform in size, color and structure and intact z. pellucida, could be transferred in the recipients immediately or after cryopreservation. The success of this technology depends on many factors, but among the foremost are: reproductive health of animals, type and series of hormones for superovulation, the degree of synchronization estrus cycles between the donor and recipient etc. The aim of this review is to present the current situation in the embryo transfer industry worldwide and our country, and accounts consider the benefits and possible drawbacks of conventional MOET
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