Use of Ion-Channel Modulating Agents to Study Cyanobacterial Na<sup>+</sup> - K<sup>+</sup> Fluxes
2004
Pomati Francesco | Burns Brendan P. | Neilan Brett A.
Here we describe an experimental design aimed to investigate changes in total cellular levels of Na<sup>+</sup> and K<sup>+</sup> ions in cultures of freshwater filamentous cyanobacteria. Ion concentrations were measured in whole cells by flame photometry. Cellular Na<sup>+</sup> levels increased exponentially with rising alkalinity, with K<sup>+</sup> levels being maximal for optimal growth pH (~8). At standardized pH conditions, the increase in cellular Na<sup>+</sup>, as induced by NaCl at 10 mM, was coupled by the two sodium channel-modulating agents lidocaine hydrochloride at 1 &mgr;M and veratridine at 100 &mgr;M. Both the channel-blockers amiloride (1 mM) and saxitoxin (1 &mgr;M), decreased cell-bound Na<sup>+</sup> and K<sup>+</sup> levels. Results presented demonstrate the robustness of well-defined channel blockers and channel-activators in the study of cyanobacterial Na<sup>+</sup>- K<sup>+</sup> fluxes.
اظهر المزيد [+] اقل [-]الكلمات المفتاحية الخاصة بالمكنز الزراعي (أجروفوك)
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