New method of identification of I and III collagen types at forming fibril framework of mesonephros and metanephros of human embryos
2009
Boyko T.A. | Shapovalova Ye.Yu.
Becoming of collagen composition of fibril framework of meso- and metanephros was studied by the method of immunohistochemistry and lectinochistochemistry on 124 human embryos in age from 21 days to 12 weeks of intrauterine development. Embryos were fixed in formalin and embedded in the paraffin. Primary antibodies were monoclonal antibodies to the collagen type I and III. Lectins of Perca fluviatilis and Vica Alba were used. It is received, that collagen fibers type I were stained by lectin of Perca fluviatilis and collagen fibers type III were stained by lectin of Vica Alba. In 12 weeks em-bryo mesonephros connective tissue fibers consisted of types I and III collagen. In 12 weeks aged embryo metanephros stroma connective tissue fibers consisted of types I and III collagen. Collagen fibers of III type firstly appeared in meso- and metanephros. Type I collagen fibers are found later. The collagen fibers type I repeated a dynamics and histotopography of collagen fibers type III, but them far fewer.
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