EFFECTS OF MATURATIONAL HORMONE TREATMENT ON SPERMATOGENESIS  OF HYBRID CATFISH (Clarias macrocephalus x C. gariepinus)

A. B. ABOL- MUNAFI, P. T. LIEM, M. A. AMBAK; S. S. SIRAJ

EFFECTS OF MATURATIONAL HORMONE TREATMENT ON SPERMATOGENESIS OF HYBRID CATFISH (Clarias macrocephalus x C. gariepinus)

2006

A. B. ABOL- MUNAFI, P. T. LIEM, M. A. AMBAK | S. S. SIRAJ

Hybridization between two different species for the same group of fishes may produce fertile hybrid F1. However, in hybrid catfish (Clarias macrocephalus x C. gariepinus) the females are fertile but hybrid males can not be used in producing F2 and backcross hybrid. In order to improve the spermatogenesis, a series treatment with sinlge hormone of ovaprim,ovaplant, human chorionic gonadotropin (hCG), carp pituitary gland (cPG) and combination of cPG with ovaprim or hCG were tested. Gonado- (GSI) and seminal vesicle-somatic index (SVSI), fertility and histological observation were used to evaluate changes of testis. The GSI increased (p<0.05) after treated with cPG pr ovaplant. Treatment with ovaplant or hCG also increased the SVSI. However, there was no change in all treatment using combination of cPG and hCG or ovaprim. Histological observation showed 25% of total observed testes producing a few sperm, but backcross with eggs of C. macrocephalus still gave negative result.

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