Isolation and identification of fungai microbiota and of dermatophytes irom healthy horses and from dermatophytosis -affected horses | Isolamento e identificação da microbiota fúngica e de dermatófitos da pele de eqüinos hígidos e daqueles afetados por dermatofitose

Due to the scarcity of reports, in Lafin-American and Brazilian literature, related to fungal microbiota of healthy horses, or to the main species of dermatophytes in horses showing suggestive lesions of dermatophytic infections, 175 horses of both sexes and different ages, including purebred horses, crossbreed, or others with no specified breed were divided in 2 groups. Group 1 was constituted by 133 asymptomatic horses without any cutaneous lesions; after dermatologie examinations, horses were submitted to Wood’s Light (48 horses), samples taken by “carpets technique” and later cultivated in Agar Sabouraud Dextrose, Mycobiotic Agar, Trichophyton Agar 3, Trichophyton Agar 5, at temperature of 25°C and 37°C during 30 days. The fungal species isolated were Pénicillium sp (80.4%), Rhizopus sp (62.4%), Aspergillus sp (41.3%), Fusarium sp (40.6%), Cladosporium sp (33.1%), Trichoderma sp (21.0%), Mucor sp (18.0%), Epicoccum sp (12.0%), Mycelia sterillia (8.8%), Rhodotorula sp (7.5%), Neurospora sp (4.5%), Alternaria sp (3.7%), Aureobasidium sp (3.7%), Geotrichum sp (3.0%), Paecilomyces sp (2.2%), Monascus sp (2.2%), Cephalosporium sp (1.5%), Nigrospora sp (0.7%), Scopulariopsis brevicaulis (0.7%) and Trichosporon sp (0.7%). Group 2 was represented by 42 horses presenting suggestive lesions of dermatophytosis, and after being submitted to dermatologie examination and to Wood’s Light (22 horses), hairs and scales or crusts were then cultured. From these, 6 (14.3%) horses were positive for fungal growth identified as Dermatophylus congolensis, and from 3 (7.1%) other horses, Microsporum<br />canis was isolated. Of the 70 equines exposed to Wood’s Light, false fluorescence was found in 2 horses.

Pela inexistência na literatura latino-americana e brasileira de trabalhos que enfoquem a microbiota fúngica de eqüinos hígidos e as principais espécies de dermatófitos em casos de eqüinos com lesões sugestivas de infecção dermatofítica do tegumento cutâneo, utilizaram-se 175 eqüinos, de ambos os sexos, tanto de raça definida como daqueles sem perfeita definição racial, de diferentes idades e que foram reunidos em 2 grupos. O GRUPO I composto de 133 eqüinos assintomáticos e desprovidos de lesões cutâneas, dos quais, após exame dermatológico, interposição<br />da Luz de Wood (48 eqüinos), colheram-se, pela técnica do carpete, material que foi semeado em meios de ágar Sabouraud Dextrose, Mycobiotic ágar, Tricophyton ágar 3, Tricophyton ágar 5 e incubados a 25° e 37°C durante 30 dias. Isolaram-se: Penicillium sp (80,4%), fíhizopus sp (62,4%), Aspergillus sp (41,3%), Fusarium sp (40,6%), Cladosporium sp (33,1%), Trichoderma sp (21,0%), Mucorsp (18,0%), Epicoccum sp (12,0%), Mycelia sterillia (8,8%), Rhodotorula sp (7,5%), Neurospora sp (4,5%), Alternaria sp (3,7%), Aureobasidium sp (3,7%), Geotrichum sp (3,0%), Paecilomyces sp (2,2%), Monascus sp (2,2%), Cephalosporium sp (1,5%), Nigrospora sp (0,7%), Scopulariopsis brevicaulis (0,7%), Trichosporon sp (0,7%). O GRUPO II foi composto por 42 eqüinos portadores de lesões sugestivas de dermatofitose que, após terem sido submetidos a exame dermatológico, expostos à luz de Wood (22 eqüinos), tiveram pelame e crostas submetidos a cultivo micológico, isolando-se em 6 (14,3%) cepas de Dermatophylus congolensis e<br />em 3 (7,1%) eqüinos houve o crescimento de dermatófitos da espécie Microsporum canis. Dos 70 eqüinos expostos à radiação ultravioleta observou-se falsa fluorescência em 2 animais.<br />