Molecular epidemiology of Listeria monocytogenes isolated from different sources in Brazil

Andrea Micke Moreno; Renata Paixão; Luisa Zanolli Moreno; Débora Dirani Sena de Gobbi; Daniele Cristine Raimundo; Thais Sebastiana Porfida Ferreira; Ernesto Hofer; Maria Helena Matte; Marina Moreno

Molecular epidemiology of Listeria monocytogenes isolated from different sources in Brazil | Epidemiologia molecular de Listeria monocytogenes isoladas de diferentes fontes no Brasil

2013

<p class="MsoNormal"><span style="mso-ansi-language: EN-US;" lang="EN-US">Listeria monocytogenes is an important foodborne pathogen that primarily affects pregnant women, neonates, the elderly and immune-compromised individuals, and it may cause abortion, septicemia, and meningitis. From the 13 capsular groups described, serotypes 4b, 1/2b and 1/2a are most closely related to human infection. For this reason, serotyping has limited value as an epidemiological tool; thus, improved discriminatory typing methods are required to enhance knowledge of L. monocytogenes contamination and infection. The aim of this study was to characterize the genetic diversity of L. monocytogenes isolates in the pork processing industry in Sao Paulo, Brazil and human infection isolates by ERICPCR and single enzyme AFLP. Serotypes 1/2c and 4b were frequent among isolates from pork and slaughterhouse/market environments, whereas serotypes 4b and 1/2a were observed among human isolates. ERIC-PCR and AFLP revealed 34 and 31 distinct profiles, respectively, which had tendencies of separation according to serogroup and isolate origin. The genetic profiles from slaughterhouse and market environments suggest the possibility of different sources of Listeria contamination in the environment, although in certain cases, continuous contamination caused by the persistence of clonal strains is also a possibility.</span></p>

اظهر المزيد [+]

<p class="MsoNormal">Listeria monocytogenes é um importante patógeno de origem alimentar que afeta principalmente grávidas, neonatos, idosos e indivíduos imunocomprometidos, e pode causar abortamento, septicemia e meningite. Dos 13 grupos capsulares descritos, os sorotipos 4b, 1/2b e 1/2a são os mais relacionados à infecção humana. Por esta razão, a sorotipagem possui valor limitado como ferramenta epidemiológica e, dessa forma, métodos mais discriminatórios são necessários para melhorar o conhecimento sobre a contaminação e a infecção por L. monocytogenes. O objetivo deste estudo foi caracterizar a diversidade genética de isolados de L. monocytogenes da indústria de processamento de carne suína no Estado de São Paulo, Brasil, e compará-los a isolados de casos de infecção humana através do ERIC-PCR e AFLP com uma única enzima. Os sorotipos 1/2c e 4b foram frequentes em carne suína e ambientes de abatedouros e mercados, enquanto os sorotipos 4b e 1/2a foram observados nos isolados de humanos. ERIC-PCR e AFLP resultaram em 34 e 31 perfis distintos, respectivamente, com uma tendência a separar de acordo com o sorogrupo e a origem do isolado. Os perfis genéticos de ambiente dos abatedouros e mercados sugerem a possibilidade de diferentes origens de contaminação por Listeria nos ambientes estudados, porém, em alguns casos, é possível que ocorra a persistência de cepas clonais causando contaminação contínua.</p>

اظهر المزيد [+]

الكلمات المفتاحية الخاصة بالمكنز الزراعي (أجروفوك)

aflp listeria monocytogenes saúde pública

المعلومات البيبليوغرافية

نُشرت في

Brazilian Journal of Veterinary Research and Animal Science

المجلد 50 الإصدار 2 الرقم التسلسلي المعياري الدولي (ردمد) 1413-9596 | 1678-4456

الناشر

Universidade de São Paulo. Faculdade de Medicina Veterinária e Zootecnia

ترقيم الصفحات

p.136-144

مواضيع أخرى

Eric-pcr

اللغة

البرتغالية

في أجريس منذ: 2013-06-15

نوع الملف: AGRIS AP

مزود البيانات

تم تزويد هذا السجل من قبل Universidade de São Paulo

اكتشف مجموعة مزود البيانات هذا في أجريس

الروابط

http://revistas.usp.br/bjvras/article/view/61229

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Molecular epidemiology of Listeria monocytogenes isolated from different sources in Brazil | Epidemiologia molecular de Listeria monocytogenes isoladas de diferentes fontes no Brasil

2013

الكلمات المفتاحية الخاصة بالمكنز الزراعي (أجروفوك)

المعلومات البيبليوغرافية