Transplantation of goat bone marrow mesenchymal stem cells (gmscs) help restore spermatogenesis in endogenous germ cells-depleted mouse models
2012
Wang Fang, Northwest A and F University, Yangling (China), College of Veterinary Medicine, Shaanxi Centre of Stem Cells Engineering and Technology | Liu Chao, Northwest A and F University, Yangling (China), College of Veterinary Medicine, Shaanxi Centre of Stem Cells Engineering and Technology | Zhang Shanshan, Northwest A and F University, Yangling (China), College of Veterinary Medicine, Shaanxi Centre of Stem Cells Engineering and Technology
Mesenchymal stem cells (MSCs) derived from bone marrow are a well-characterized population of adult stem cells that can be maintained and propagated in culture for a long time with the capacity to form a variety of cell types. This study investigated the characteristics of dairy goat bone marrow MSCs (gMSCs) and their differentiation potential toward germ cells in vitro, and to test their potential in vivo, these cells were transplanted into seminiferous tubes of endogenous germ cells-depleted mouse models. The results showed that characteristic gMSC lines were established and a small population of gMSCs transdifferentiated into male germ cell-like cells which expressed Stra8 after induction with retinoic acid (RA), as analysed by reverse transcription-polymerase chain reaction (RT-PCR) and immunofluorescence. Further, we transplanted the gMSCs into endogenous germ cells-depleted mouse models. A variety of analysis demonstrated that gMSCs might differentiate into male germ cells and helped spermatogenesis in endogenous germ cells depleted mouse models at 30 d after transplantation. The gMSCs could be used as a potential source of cells for reproductive studies and a neoadjuvant therapy for the spermatogenesis anomaly. Moreover, these cells may offer a new strategy for male infertility and an alternative approach for production of transgenic animals.
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