The role of cytokinins during micropropagation of wych elm
2013
Mala, J., Forestry and Game Management Research Inst., Strnady (Czech Republic) | Machova, P., Forestry and Game Management Research Inst., Strnady (Czech Republic) | Cvrckova, H., Forestry and Game Management Research Inst., Strnady (Czech Republic) | Karady, M., Palacky Univ., Olomouc (Czech Republic). Lab. of Growth Regulators | Novak, O., Palacky Univ., Olomouc (Czech Republic). Lab. of Growth Regulators | Mikulik, J., Palacký University and Institute of Experimental Botany AS CR, Olomouc-Holice, (Czech Republic). Lab. of Growth Regulators | Dostal, J., Forestry and Game Management Research Inst., Strnady (Czech Republic) | Strnad, M., Palacky Univ., Olomouc (Czech Republic). Faculty of Science | Dolezal, K., Palacky Univ., Olomouc (Czech Republic). Faculty of Science
We have compared the influence of two aromatic cytokinin derivatives, N6-benzyladenine (BA) and meta-topolin (mT), on the in vitro multiplication and senescence of Ulmus glabra. After 3 months of cultivation, the micropropagation rate was higher (approx. six times more shoots developed) on Murashige and Skoog (MS) medium supplemented with mT than on MS supplemented with BA. Quantification of 50 endogenous cytokinins, using a recently developed UPLC-ESI(+)-MS/MS method, showed significant differences in the cytokinin metabolites (especially different glucosides) in explants cultivated on media supplemented with BA or mT. Differences in efficiency of photosystem II was also detected.
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