Molecular genetic studies on avermectin production in some microorganisms
2011
Ali, R.S.M.
Avermectins, are important secondary metabolites produced by soil bacterium called Streptomyces avermitilis. These compounds are important in animal, human health and crop protection. The broad spectrum agricultural pesticidal activity of the avermectins was demonstrated against insect pests in several orders. The killing ability of S. avermitilis NRRL 8165 against plant parasite nematodes improved by the Induction of mutation in parental strain Streptomyces avermitilis NRRL 6185 using ethedium bromide as a mutagen, to obtain new recombinants differ in their ability to produce avermectin compounds and in their morphological and physiological character comparing to the wild type. Results from mutation indicated that the growth rate in two mutants accelerated by significant value, where the growth of them appeared after 2 days and 4 days compared with 7 days growth in parental strain NRRL 6185. The production of avermectin complex was detected in the both of fermentation medium of both parental strain S. avermitilis NRRL8165 and mutants by detecting the killing ability of each stain on nematode larvae, and chromatographically using thin layer chromatography assay (TLC).
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