Embryo production in buffaloes, Bubalus bubslis, with some supplementation in superovulatory regimens
2011
Nasra, A.A. | Badr, M.R. | Darwish, G.M.
The aim of this study is to evaluate the effect of different superovulatory regimens on embryo production of buffalo-cows in Egypt. Estrus synchronization applied on 16 multiparous Egyptian buffalo-cows divided randomize into three groups A (n=4), B (n=6) and C (n=6). Only, thirteen buffalo cows responded in the three groups A (n=3); B (n=4) and C (n=6). The buffaloes showed estrus from the three groups were received a dose of 2500 IU of eCG on day 8 of the cycle. In group A (n=3) no, further superovulatory treatment was applied, whereas animals of group B (n= 4) received a single dose of 2500 IU of hCG 30 h after AI. Buffaloes of group C (n= 6) received the same regimen of group B in addition to I.m injection of 2.5 mg Estradiol benzoate (EB) and 50mg progesterone (P4) 4 days before superovulatory treatments. Embryos were collected from all groups non- surgically during day 6 (around 140) h from estrus onset. The results reveals that there were a significance increase (P0.05) for the number of corpora lutea and collected embryos in group C than other treated groups. In conclusion, progesterone supplementation with estradiol benzoate 4 days before initiating the superovulation treatment seems to enhance the recovery rate in buffaloes. Further studies are needed in order to increase the number of embryos recovered per a buffalo female.
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