Evaluation of Fermentation Ability of Microbes for Corn Silage Inoculant
2010
Kim, J.G., National Institute of Animal Science, RDA, Cheonan, Republic of Korea | Ham, J.S., National Institute of Animal Science, RDA, Cheonan, Republic of Korea | Chung, E.S., National Institute of Animal Science, RDA, Cheonan, Republic of Korea | Seo, S., National Institute of Animal Science, RDA, Cheonan, Republic of Korea | Park, H.S., National Institute of Animal Science, RDA, Cheonan, Republic of Korea
Corn is very important forage in Korea. The great part of them is utilized as silage. Generally, it contains a lot of grains that is feed of animal. This experiment was conducted to evaluation of fermentation ability of microbes for com silage inoculant. Good lactic acid bacteria were isolated from good com silage by plating MRS agar containing 0.02% sodium azide, and assessed by growing and acid producing ability in MRS broth. Six lactic acid bacteria were selected, and were found to be Gram positive, rods and catalase negative and were identified to be lactobacillus plantarum (C3-2, B13-1, CC9-1), Lactobacillus fermentum(C11-4), Lactobacillus paracasei(B14-1), and Leuconostoc lactis (A3-1) on the basis of the biochemical characteristics and utilization of substrates. Com was ensiled at ripen stage following treatment with selected five lactic acid bacteria, two commercial inoculant, and no additive (control). After 2 month, B13-1 and CC9-1 bacteria inoculated silage were lower pH and higher lactic acid content than others treatments. The Flieg's score and grade of B13-1 and CC9-1 bacteria treated silage were higher than commercial inoculant. According to this experiment, lactobacillus plantarum B13-1 and CC9-1 strain were recommendable for good inoculant of com silage.
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