Biological Activity of Organic Solvent Fractions from Lythrum salicaria L. (Root)
2010
Lee, S.E., Department of Herbal Crop Research, NIHHS, RDA, Eumsung, Republic of Korea | Kim, G.S., Department of Herbal Crop Research, NIHHS, RDA, Eumsung, Republic of Korea | Han, H.S., Department of Herbal Crop Research, NIHHS, RDA, Eumsung, Republic of Korea | Lee, E.S., Department of Herbal Crop Research, NIHHS, RDA, Eumsung, Republic of Korea | Kim, Y.O., Department of Herbal Crop Research, NIHHS, RDA, Eumsung, Republic of Korea | Lee, J.H., Department of Herbal Crop Research, NIHHS, RDA, Eumsung, Republic of Korea | Seong, N.S., International Ginseng and Herb Research Institute, Geumsan, Republic of Korea | Lee, S.W., Department of Herbal Crop Research, NIHHS, RDA, Eumsung, Republic of Korea | Kim, Y.C., Department of Herbal Crop Research, NIHHS, RDA, Eumsung, Republic of Korea
Root extract of Lythrum salicaria reported a hepato-protective effect on CCl₄-induced liver toxicity of rat was prepared into fractions such as n-hexane up layer (HA), n-hexane down layer (HB), diethyl ether (E), ethylacetate (EA), n-butanol (B) and water (W). Fractions prepared were tested their activities in vitro and in vivo condition. All of the fractions showed effective antioxidant asctivities on DPPH radical and CuSO₄-induced oxidation of human low density lipoprotein and E fraction showed the highest inhibitory effect (98.1% at 50 ㎍/㎖) on linoleic acid autoxidation at 40℃, which was more effective than α-tocopherol (82.4%). Five fractions (H = HA plus HB, E, EA, B, and W, 150 mg/kg/day) were fed into Sprague Dawley, male rats for 4 days, which were intoxicated with intra-peritoneal injection of carbon tetrachloride (1 ㎖/kg in corn oil) at the 4th day and were sacrificed in 24 hrs. Serum tumor necrosis factor-alpha (TNF-α), a proinflammatory cytokine, elevated with CCl₄-intoxication in negative control group (83 pg/㎖) was significantly decreased in E fraction-supplemented group (18 pg/㎖). Cu, Zn-superoxide dismutase (SOD) activity increased in negative control group (0.12 U/mg protein) was decreased in E fraction (0.07 U/mg protein). From the results, it is suggested that ether fraction from root extract of L. salicaria would be a potent antioxidant candidate for ameliorating liver injury induced by chemical intoxicant.
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