The Early Detection of the Spore Using Sonication and Fluorescent Dye in the Field
2011
Ha, Y.C., The 5th Institute-4, Agency for Defense Development, Daejeon, Republic of Korea | Choi, K.B., The 5th Institute-4, Agency for Defense Development, Daejeon, Republic of Korea
This study was carried out to establish the optimum condition of cell disruption with a sonicator for the detection of the spore, Bacillus anthracis Δ-sterne for the purpose of developing automatic fluorometer. The efficiency of sonication on the Δ-sterne spore disruption was very weak. The Δ-sterne spore with zirconia bead showed greater disruption than the Δ-sterne spore alone when sonificated. The volumn of the zirconia bead added in the spore solution has little effect on the disruption efficiency. The detection limit of the Δ-sterne spore with zirconia bead and the Δ-sterne spore alone was 10∨6 CFU/mL and 5×10∨7 CFU/mL respectively, when sample was sonicated for 20 seconds with a sonicator probe of 13 mm diameter.
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