Identification of variations in the alfa-lactoalbumin gene in cow population of Latvia | Alfa-laktoalbumīna (α-LA) gēna variāciju identificēšana un tā analīze Latvijas govju populācijā
2012
Smiltina, D., Latvia Univ. of Agriculture, Jelgava (Latvia). Faculty of Agriculture. Inst. of Agrobiotechnology. Lab. of Molecular Genetic Research | Balins, A., Latvia Univ. of Agriculture, Jelgava (Latvia). Faculty of Agriculture. Inst. of Agrobiotechnology. Lab. of Molecular Genetic Research | Grislis, Z., Latvia Univ. of Agriculture, Jelgava (Latvia). Faculty of Agriculture. Inst. of Agrobiotechnology
The aim of this study was oriented to the identification of α - lactoalbumin gene (α-LA) polymorphism and to analysis of genotype structure in population of the dairy cattle in Latvia. The experimentation was carried out at the Laboratory of Molecular Genetic Research of the Faculty of Agriculture of Latvia University of Agriculture. As a research material of 123 blood and 70 sperm cells samples from 7 breeds where used. The PCR products were digested with the restriction enzyme Mnll. Alleles A and B of α-LA were distinguished by Polymerase Chain Reaction and Restriction Fragment Length Polymorphism (PCR – RFLP) analysis. Frequency of α-LA allele B is from 0.7073 to 0.9667. This research is a novelty in Latvia.
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