Biochemical Characterization of Thermophilic Dextranase from a Thermophilic Bacterium, Thermoanaerobacter pseudethanolicus
2012
Park, T.S., Infection Control Material Research Center, KRIBB, Jeongeup, Republic of Korea | Jeong, H.J., Infection Control Material Research Center, KRIBB, Jeongeup, Republic of Korea | Ko, J.A., Infection Control Material Research Center, KRIBB, Jeongeup, Republic of Korea | Ryu, Y.B., Infection Control Material Research Center, KRIBB, Jeongeup, Republic of Korea | Park, S.J., Infection Control Material Research Center, KRIBB, Jeongeup, Republic of Korea | Kim, D.M., Chonnam National University, Gwangju, Republic of Korea | Kim, Y.M., Infection Control Material Research Center, KRIBB, Jeongeup, Republic of Korea | Lee, W.S., Infection Control Material Research Center, KRIBB, Jeongeup, Republic of Korea
TPDex, a putative dextranase from Thermoanaerobacter pseudethanolicus, was purified as a single 70 kDa band of 7.37 U/mg. Its optimum pH was 5.2 and the enzyme was stable between pH 3.1 and 8.5 at 70℃. A half-life comparison showed that TPDex was stable for 7.4 h at 70℃, whereas Chaetominum dextranase (CEDex), currently used as a dextranase for sugar milling, was stable at 55℃. TPDex showed broad dextranase activity regardless of dextran types, including dextran T2000, 742CB dextran, and alternan. TPDex showed the highest thermostability among the characterized dextranases, and may be a suitable enzyme for use in sugar manufacture without decreased temperature.
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