Detection of feline calicivirus as norovirus surrogate in food and water sources using filtration and real-time RT-PCR
2011
Cho, M.G., Chungbuk National University, Cheongju, Republic of Korea | Jeong, H.M., Chungbuk National University, Cheongju, Republic of Korea | Ahn, J.B., Seowon University, Cheongju, Republic of Korea | Kim, K.Y., Chungbuk National University, Cheongju, Republic of Korea
Norovirus (NoV) is a major cause of acute non-bacterial gastroenteritis in all age groups worldwide. To detect NoV from foods, polyethylene glycol (PEG) precipitation or ultracentrifugation methods are generally used with reverse transcription (RT)-PCR. These methods need to use complicated procedures and varied buffers depending on the kinds of food matrices. In this study, we suggested a universal method to recover NoV in food and water samples as a prior step to real-time RT-PCR. As a NoV surrogate model, feline calicivirus (FCV) was used. FCV was artificially inoculated to samples, and then concentrated by the adsorption-elution method using negatively charged membrane filters. The detection limit was 4.3×10¹ PFU/250 mL for distilled water, 4.3×10² PFU/250 mL for environmental waters, and 4.3×10² PFU/15 g for lettuce and oyster. We were able to identify the possibility of one universal and time-saving method to detect NoV in food and water samples without modifications.
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