Antiviral Peptide from Pseudomonas chlororaphis O6 against Tobacco Mosaic Virus (TMV)
2012
Park, J.Y., Chonnam National University, Gwangju, Republic of Korea | Yang, S.Y., Chonnam National University, Gwangju, Republic of Korea | Kim, Y.C., Chonnam National University, Gwangju, Republic of Korea | Kim, J.C., Korea Research Institute of Chemical Technology, Daejeon, Republic of Korea | Dang, Quang Le, Korea Research Institute of Chemical Technology, Daejeon, Republic of Korea | Kim, J.J., National Academy of Agricultural Science, RDA, Suwon, Republic of Korea | Kim, I.S., Chonnam National University, Gwangju, Republic of Korea
Although Pseudomonas chlororaphis O6 (O6) is known to be a rhizobacterium capable of inducing systemic resistance against plant virus, its antiviral products from O6 remain unknown. In the present study, an antiviral cyclic peptide was identified from the cell-free supernatant of O6. O6 cultures grown on Luria Bertani medium were centrifuged, and the resulting supernatant was extracted with organic solvent, followed by a series of column chromatography and preparative high performance liquid chromatography (HPLC). Bioassay-guided fractionations were involved in the isolation of antiviral products against tobacco mosaic virus (TMV). Time of flight mass spectrometry (TOF-MS) analysis of the isolated product detected (M+H)+ peak at m/z 887.4242 that generated m/z 756.3859, 657.3180, 556.2724, 459.2208, 345.1873, and 171.1130 as the main fragment ions. NMR analyses characterized all protons and carbons of the isolated product. Based on the data, the isolated antiviral product was determined to be a cyclic peptide with molecular formula C∧39H∧67N∧9O∧12S consisting of seven different amino acids. The antiviral peptide exhibited more than 95% disease suppression of TMV at 1,000 ㎍/mL. O6 may play a role in promoting plant growth.
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