Inhibition of Xanthine Oxidase by Phenolic Phytochemicals from Broussonetia papyrifera
2012
Ryu, H.W., Graduate School of Gyeongsang National University, Jinju, Republic of Korea | Lee, J.H., Graduate School of Gyeongsang National University, Jinju, Republic of Korea | Kang, J.E., Graduate School of Gyeongsang National University, Jinju, Republic of Korea | Jin, Y.M., Graduate School of Gyeongsang National University, Jinju, Republic of Korea | Park, K.H., Graduate School of Gyeongsang National University, Jinju, Republic of Korea
The roots of Broussonetia papyrifera were extracted into four different polar solvents: chloroform, 50% ethanol in water, ethanol, and water. The ethanol extract showed the most potent inhibition (72.3% at 20 g/mL) against xanthine oxidase (XOD). Chromatography of EE yielded nine phenolic phytochemicals, which were confirmed as broussochalcone A (1), broussochalcone B (2), 3,4-dihydroxyisolonchocarpin (3), 4-hydroxyisolonchocarpin (4), 3-'(3-methylbut-2-enyl)-3',4',7-trihydroxyflavane (5), kazinol A (6), kazinol B (7), kazinol E (8), and broussoflavan A (9). All isolated compounds (19) possessed potent antioxidant activities against 2,2-diphenyl-l-picrylhydrazyl and 2,2'-azino-bis-ethyl-benzthiazoline-6-sulfonic acid (ABTS) radicals with IC∧50 values ranging from 5.8 to 252.8M. Although most compounds exhibited potent inhibition with IC∧50 values ranging 0.6-164 M against XOD, compounds 1 and 3 were found to be the principal contributors to the XOD inhibition in ethanol extract. The analysis of K∧I and K∧IS values proved that the two most promising compounds (1 and 3), present at high concentrations in the root barks as analyzed by using high-performance liquid chromatography analysis, were reversible mixed type I inhibitors.
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