Characterization of Protease Produced by Elizabethkingia meningoseptica CS2-1 and Optimization of Cultural Conditions for Amino Acid Production
2011
Kim, S.J., Mokwon University, Daejeon, Republic of Korea | Cho, C.H., KAFCO Biochemistry Research Institute, Okcheon, Republic of Korea | Whang, K.S., Mokwon University, Daejeon, Republic of Korea
A feather-degrading bacterium Elizabethkingia meningoseptica CS2-1 was isolated from compost in a chicken farm. Cultured on a basal medium containing 2% chicken feather, the bacterium showed 729.7 μmol/mL of amino acid. Optimal culture conditions for feather degradation by E. meningoseptica CS2-1 were 25℃, pH 7.5, and 180 rpm. The optimal pH and temperature for protease activity were 8.0 and 40℃, respectively. The composition of an optimal medium for amino acid production was 0.05% NH4Cl, 0.05% NaCl, 0.03% K₂HPO₄, 0.03% KH₂PO₄, 0.01% MgCl₂ㆍ6H₂O, 0.1% urea, and 2% chicken feather. Characteristics of amino acids extracted from the optimal medium under the optimal culture conditions of E. meningoseptica CS2-1 were analyzed. The total amino acid content of strain CS2-1 was 1063 μmol/mL, which was 46% higher compared to the basal condition (729.7 μmol/mL). The essential amino acid content in the total amino acid was 315.9 μmol/mL, which was 44% higher than that of the basal condition. Major amino acids were proline (14%), aspartic acid (12%), glutamic acid (11%), serine (10%), alanine (10%), glycine (9%), and tyrosine (7%) by strain CS2-1. These results suggest that strain CS2-1 can be used as a potential microbial resource for the production of amino acid using chicken feathers.
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