Effect of some in vitro propagation methods on production of citrus plants having good vegetative and reproductive characters

This investigation was carried out during the seasons of 2006 to 2009 on three cultivars ýof citrus namely Valencia orange (Citrus sinensis), Navel orange Var. Washington ýý(Citrus sinensis) and Balady Mandarin (Citrus deliciosa Tan.), parts of immature fruits ýincluded juice vesicle and albedo tissues as explants to study the effect of cultivars, ýexplants and nutrient media on in vitro growth and development of these citrus cultivars. ýData revealed that sterilizing the explants by 20% Clorox for 15 min. and 70% ethanol ýgave 100% recovered explants on both studied seasons. This experiment was carried out ýto study the effect of MS medium with 2, 4-D at 0.0, 0.5, 1, 2 or 3 mg/l, activated ýcharcoal at 1 g/l. and kinetin at concentrations of 0.00, 0. 10, 0.25, 0.50 or 1.00 mg/I for ýembryogenesis. MS medium supplemented with BA at concentrations of 0.5, 1.0, 2.0 or ýý4.0 mg/l, NAA at concentrations of 0.25 or 0.5 mg/I and GA3 at concentrations of 0.5 or ýý1.0 mg/1 for adventitious shoot induction MS medium supplemented with NAA at ýconcentrations of 0.5 or 2.0 mg/I, IBA at concentrations of 0.5 or 2.0 mg/1 and BA at ýý0.25 mg/I for rooting. The acclimatization has done in a mixture of sand: peat moss: ýcompost (1:1:1, v:v:v). MS media with 2,4-D at 3 mg/l. with kinetin at 0.1 mg/I. ýsignificantly increased the callused explants and callus diameter. MS media with 2,4-D at ýý1 mg/l. and kmetin at 0.25 mg/l. significantly increased embryo length and embryo ýnumber/explant. MS media with BA at 2 mg/1, NAA at 0.5 mg/1 and GA3 at 0.5 mg/l. ýsignificantly increased the shoot number/explant and the leaves number/explant. Also, ýMS with BA at 0.5 mg/l, NAA at 0.25 mg l. GA3 at I mg/I. significantly increased the ýshoot length. MS media supplemented with NAA at 2 mg/I., IBA at 2 mg/I. and BA at ýý0.25 mg/l. significantly increased the root number/explant. MS media supplemented with ýNAA at 0.5 mg/l., IBA at 0.5 mg/I. and BA at 0.25 mg/i. significantly increased the root ýlength. The results of acclimatization were 80-85% success, 8.00 leaves/plantlet and 30.0 ýcm stem length. ý