Analysis of virion structural components reveals vestiges of the ancestral ichnovirus genome
2010
Volkoff , Anne Nathalie (INRA , Montpellier (France). UMR 1231 Biologie Intégrative et Virologie des Insectes) | Jouan , Véronique (INRA , Montpellier (France). UMR 1231 Biologie Intégrative et Virologie des Insectes) | Urbach , Serge (Centre National de la Recherche Scientifique, Montpellier(France). Institut de Génomique Fonctionnelle, Plate-forme Protéomique, CNRS UMR 5203, INSERM U661) | Samain , Sylvie (Commissariat à l'Energie Atomique et aux Energies Alternatives, Evry(France). IG (Genoscope), Centre National de Séquençage) | Bergoin , Max (INRA , Montpellier (France). UMR 1231 Biologie Intégrative et Virologie des Insectes) | Wincker , Patrick (Commissariat à l'Energie Atomique et aux Energies Alternatives, Evry(France). IG (Genoscope), Centre National de Séquençage) | Demettre , Edith (Centre National de la Recherche Scientifique, Evry(France). IG (Genoscope), Centre National de Séquençage) | Cousserans , Francois (INRA , Montpellier (France). UMR 1231 Biologie Intégrative et Virologie des Insectes) | Provost , Bertille (INRA , Montpellier (France). UMR 1231 Biologie Intégrative et Virologie des Insectes) | Coulibaly , Fasseli (Monash University, Melbourne(Australie). Structural Virology Group, Department of Biochemistry & Molecular Biology) | Legeai , Fabrice (INRA , Le Rheu (France). UMR 1099 Biologie des Organismes et des Populations Appliquée à la Protection des Plantes) | Béliveau , Catherine (Natural Resources Canada, Québec(Canada). Laurentian Forestry Centre) | Cusson , Michel (Natural Resources Canada, Québec(Canada). Laurentian Forestry Centre) | Gyapay , Gabor (Commissariat à l'Energie Atomique et aux Energies Alternatives, Evry(France). IG (Genoscope), Centre National de Séquençage) | Drezin , Jean-Michel (Centre National de la Recherche Scientifique, Tours(France). UMR 6035, Institut de Recherche sur la Biologie de l’Insecte, UFR des Sciences et Techniques, Universite´ Franc¸ois Rabelais, Parc Grandmont)
Many thousands of endoparasitic wasp species are known to inject polydnavirus (PDV) particles into their caterpillar host during oviposition, causing immune and developmental dysfunctions that benefit the wasp larva. PDVs associated with braconid and ichneumonid wasps, bracoviruses and ichnoviruses respectively, both deliver multiple circular dsDNA molecules to the caterpillar. These molecules contain virulence genes but lack core genes typically involved in particle production. This is not completely unexpected given that no PDV replication takes place in the caterpillar. Particle production is confined to the wasp ovary where viral DNAs are generated from proviral copies maintained within the wasp genome. We recently showed that the genes involved in bracovirus particle production reside within the wasp genome and are related to nudiviruses. In the present work we characterized genes involved in ichnovirus particle production by analyzing the components of purified Hyposoter didymator Ichnovirus particles by LC-MS/MS and studying their organization in the wasp genome. Their products are conserved among ichnovirus-associated wasps and constitute a specific set of proteins in the virosphere. Strikingly, these genes are clustered in specialized regions of the wasp genome which are amplified along with proviral DNA during virus particle replication, but are not packaged in the particles. Clearly our results show that ichnoviruses and bracoviruses particles originated from different viral entities, thus providing an example of convergent evolution where two groups of wasps have independently domesticated viruses to deliver genes into their hosts.
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تم تزويد هذا السجل من قبل Institut national de la recherche agronomique
